BACE1 抗体
Quick Overview for BACE1 抗体 (ABIN6774191)
抗原
See all BACE1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes human BACE-1. This antibody was selected for its ability to detect human or mouse BACE-1 in Western Blots and direct ELISAs. In Western Blots, no cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Species cross-reactivity: Mouse.
-
纯化方法
- Protein G purified
-
免疫原
-
Synthetic peptide from human BACE1.
Type of Immunogen: Synthetic peptide -
亚型
- IgG1
-
-
anti-Beta-secretase 1 (BACE1) (AA 22-501) antibody
BACE1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 2C1 unconjugated
anti-Beta-secretase 1 (BACE1) (AA 112-324) antibodyBACE1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3C1C3 unconjugated
anti-Beta-secretase 1 (BACE1) (N-Term) antibodyBACE1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (AA 401-501) antibodyBACE1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (AA 22-322) antibodyBACE1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (AA 46-155) antibodyBACE1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (AA 72-121) antibodyBACE1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, 猴, Pig, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (C-Term) antibodyBACE1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (N-Term) antibodyBACE1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Beta-secretase 1 (BACE1) (AA 38-70), (N-Term) antibodyBACE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB16874 unconjugated
-
-
应用备注
-
Approved: ELISA, Flo, WB (1:10000)
Usage: Western Blot-This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1. Using a colorimetric detection system, the detection limit for rhBACE-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human or mouse BACE-1 coated on 96-well plates. The detection limit for rhBACE-1 is approximately 1 ng/well. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute in 1 mL of Sterile PBS.
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from PBS, 5 % trehalose.
-
注意事项
- Aviod frequent freeze thaws.
-
储存条件
- -20 °C
-
储存方法
- Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
-
-
- BACE1 (Beta-secretase 1 (BACE1))
-
别名
- BACE1 / BACE
-
背景
-
Name/Gene ID: BACE1
Subfamily: Aspartic A1
Family: Protease
Synonyms: BACE1, APP beta-secretase, Asp 2, ASP2, Aspartyl protease 2, Aspartylprotease 2, Beta-secretase, Beta-site APP-cleaving enzyme, BACE, HSPC104, KIAA1149, B-Secretase, Beta-secretase 1, Memapsin-2 -
基因ID
- 23621
-
UniProt
- P56817
抗原
-
